OV-624 毛细管柱测卡泊三醇中甲酸残留

名称：毛细管色谱柱

固定相：6% 氰丙基苯基／94% 二甲基聚硅氧烷
性：弱性到中等性固定相
相当于 USP 固定相 G43

型号：OV-624

规格：30m*0.32mm*1.8um
应用： 毛细管气相色谱法测定卡泊三醇中甲酸残留量

滕州市浩瀚色谱仪器技术服务有限公司，建立卡泊三醇中甲酸的含量测定方法。方法使用气相色谱仪,采用OV-624毛细管色谱柱(6%氰丙基苯聚硅氧烷-94%二甲基聚硅氧烷),0.32 mm×30 m,液膜厚度为1.8μm;柱温40℃,保持5 min,以10℃.min-1的速度升温150℃;保持10 min。顶空进样,顶空瓶平衡温度:70℃,平衡时间:30 min,进样量为1 ml。以氮气为载气,流速为3 ml.min-1;FID检测器。结果甲酸线性关系良好,相关系数大于0.999;平均回收率为100.1%;检出限为1.19 mg.L-1。结论该方法简便,灵敏度高,重复性好,结果准确,适合于卡泊三醇中甲酸残留量的测定。

毛细管柱测卡泊三醇中甲酸残留 测试谱图：

Determination of formic acid residues in calcipotriol by capillary column
Determination of formic acid residues in calcipotriol by capillary column Details:

Name: Capillary chromatography column

Stationary phase: 6% cyanopropylphenyl/94% dimethyl polysiloxane
Polarity: weak polar to medium polar stationary phase
Equivalent to USP stationary phase G43

Model: OV-624

Specification: 30m*0.32mm*1.8um
Application: Determination of formic acid residue in calcipotriol by capillary gas chromatography

Tengzhou Haohan Chromatography Instrument Technology Service Co., Ltd. established a method for the determination of formic acid in calcipotriol. Methods Using a gas chromatograph, an OV-624 capillary chromatography column (6% cyanopropylbenzene polysiloxane-94% dimethyl polysiloxane), 0.32 mm × 30 m, liquid film thickness of 1.8 μm; column Keep the temperature at 40℃ for 5 minutes, and increase the temperature to 150℃ at the rate of 10℃.min-1; hold for 10 minutes. Headspace injection, headspace bottle equilibrium temperature: 70℃, equilibrium time: 30 min, injection volume is 1 ml. With nitrogen as the carrier gas, the flow rate is 3 ml.min-1; FID detector. Results The linear relationship of formic acid was good, the correlation coefficient was greater than 0.999; the average recovery was 100.1%; the detection limit was 1.19 mg.L-1. Conclusion The method is simple, sensitive, reproducible and accurate. It is suitable for the determination of formic acid residue in calcipotriol.

Capillary column measurement of formic acid residues in calcipotriol Test spectrum: